Important technical information:
The baculovirus Cydia pomonella granulosis virus and , has been developed by Bio Tepp Inc., a Canadian biopesticide company. The end use product contains a naturally occurring viral insecticide for the reduction of codling moth damage on apple trees. These products were reviewed jointly by the PMRA and the U.S. Environmental Protection Agency (EPA) under the North American Free Trade Agreement (NAFTA) Technical Working Group on Pesticides Program for Joint Review of Microbials and Semiochemicals. Microbials are increasingly being investigated for use as alternatives to conventional pesticides. is a biological insecticide containing the baculovirus Cydia pomonella granulosis virus (CpGV) (4 x 1013OBs). The guarantee for is described as 0.07% CpGV by weight or minimum of 4 × 1013 occlusion bodies (OBs)/L. Baculoviruses are arthropod-specific viruses that have been widely described and characterized in the scientific literature for close to 40 years.
The CpGV contained in has been isolated from Microbial Ecozone 4 of Canada, which encompasses the Great Lakes Region of both the U.S. and Canada, the Northeastern U.S., and the Canadian Maritimes. It is different than other CpGV-M already use in many area. Baculoviruses have a long history of safe use, and it is expected that will pose low potential risk to human health and the environment, compared with conventional pesticides. This strain is reported to be specific for the codling moth Cydia pomonella. The active ingredient, CpGV, has not been genetically modified or engineered through recombinant nucleic acid procedures.
The first application should be made at 210 degree-days (F) (100 degree-days (C*)) after biofix as determined by first consistent moth catch in pheromone traps. Diluted should be applied before egg hatching, after which it should be applied another three times at two-week intervals, for a total of two to three applications per generation.
Baculoviruses consist of a circular DNA genome surrounded by layers of protective protein molecules. Virus particles are embedded in a crystalline protein matrix called an occlusion body. These OBs are ingested when the insect larva feeds on contaminated food and dissolve in the alkaline environment of the insect midgut to release the virus particles. After penetrating the peritrophic membrane, the virus lipoprotein fuses with the plasma membrane of a gut wall cell and releases its nucleocapsid into the cytoplasm. The virus replicates within the nucleus of the host cell. Infected cells eventually rupture and release newly formed virus particles into the gut lumen where they attack other cells. The progressive increase in new virus particles and subsequent destruction of an increasing number of host gut cells eventually leads to death of the host. Virus particles are released from the decaying host into the environment where they can infect other larvae.
infection process, Sick treated larva stops feeding and dead infected larva will produce more bio-insecticide.